The inhibition of chondroitin sulphate protein synthesis by cycloheximide
نویسندگان
چکیده
منابع مشابه
Inhibition of hydroxyapatite formation in collagen gels by chondroitin sulphate.
Crystal growth in native collagen gels has been used to determine the role of extracellular matrix macromolecules in biological calcification phenomena. In this system, type I collagen gels containing sodium phosphate and buffered at pH 7.4 are overlayed with a solution containing CaCl2. Crystals form in the collagen gel adjacent to the gel-solution interface. Conditions were determined which p...
متن کاملSelective inhibition of protein synthesis initiation in Saccharomyces cerevisiae by low concentrations of cycloheximide.
We have previously determined the amounts of time required to complete various macromolecular synthetic processes needed for induction of allophanate hydrolase in Saccharomyces cerevisiae. This information provided a means of testing, in vivo, an early hypothesis suggesting that cycloheximide inhibited the initiation as well as elongation steps of protein synthesis. Our data suggest that initia...
متن کاملNature of the interaction of chondroitin 4-sulphate and chondroitin sulphate-proteoglycan with collagen.
The electrostatic interaction of chondroitin sulphate and the chondroitin sulphate-proteoglycan with collagen was studied by chromatography of the glycosaminoglycan and the proteoglycan on a collagen gel. The observed binding between the macromolecules increased with decreasing pH and ionic strength, and was significant under physiological conditions. A study of the interaction between chondroi...
متن کاملZonal distribution of chondroitin-4-sulphate/dermatan sulphate and chondroitin-6-sulphate in normal and diseased human synovium.
OBJECTIVES Chondroitin sulphate is the major sulphated glycosaminoglycan present in the extracellular matrix of soft connective tissues and the aim of this study was to investigate the distribution of chondroitin sulphate species in normal and diseased synovium. METHODS Distribution of chondroitin-4-sulphate/dermatan sulphate (Ch4S/DS) and chondroitin-6-sulphate in normal (n = 6), osteoarthri...
متن کاملMechanism of cycloheximide inhibition of protein synthesis in a cell-free system prepared from rat liver.
Sites of cycloheximide action on protein synthesis were examined using a cell-free system prepared from rat liver. I f all amino acids or aminoacyl transfer RNA were present at the start of incubation, the system appeared to incorporate W-leucine mainly by elongation of peptide chains. Under these conditions, high dose levels of cycloheximide were necessary in order to inhibit incorporation ext...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1969
ISSN: 0014-5793
DOI: 10.1016/0014-5793(69)80063-3